Pulmonary CD4+ cytokine responses in mice reveal differences in the relative immunogenicity of cold-adapted influenza A vaccine donor strains.
Identifieur interne : 001578 ( Main/Exploration ); précédent : 001577; suivant : 001579Pulmonary CD4+ cytokine responses in mice reveal differences in the relative immunogenicity of cold-adapted influenza A vaccine donor strains.
Auteurs : Mark D. Wareing [Australie] ; Leonard C. Harrison ; Gregory A. TannockSource :
- Vaccine [ 0264-410X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Basse température, Cytokines (analyse), Infections à Orthomyxoviridae (), Lymphocytes T CD4+ (immunologie), Lymphocytes T CD8+ (immunologie), Mâle, Noeuds lymphatiques (immunologie), Poumon (immunologie), Souris, Souris de lignée BALB C, Spécificité d'espèce, Vaccins antigrippaux (immunologie), Virus de la grippe A (immunologie).
- MESH :
English descriptors
- KwdEn :
- Animals, CD4-Positive T-Lymphocytes (immunology), CD8-Positive T-Lymphocytes (immunology), Cold Temperature, Cytokines (analysis), Influenza A virus (immunology), Influenza Vaccines (immunology), Lung (immunology), Lymph Nodes (immunology), Male, Mice, Mice, Inbred BALB C, Orthomyxoviridae Infections (prevention & control), Species Specificity.
- MESH :
- chemical , analysis : Cytokines.
- immunology : CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Influenza A virus, Influenza Vaccines, Lung, Lymph Nodes.
- prevention & control : Orthomyxoviridae Infections.
- Animals, Cold Temperature, Male, Mice, Mice, Inbred BALB C, Species Specificity.
Abstract
We previously described differences in the 50% protective dose and isotype-specific antibody secreting cell (ASC) responses to US and Russian influenza A cold-adapted (ca) donor strains in the lungs of BALB/c mice [Wareing MD, Watson JM, Brooks MJ, Tannock GA. Immunogenic and isotype-specific responses to Russian and US cold-adapted influenza A vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57, and A/Ann Arbor/6/60 (H2N2) in mice. J Med Virol 2001;65(1):171-7]. A/Leningrad/134/17/57(Len/17-ca) was shown to be a superior immunogen to A/Leningrad/134/47/57-ca (Len/47-ca), which, in turn, was superior to A/Ann Arbor/6/60-ca (AA-ca) but no other comparative data exist. In order to extend our findings and determine a means for selecting the most immunogenic ca influenza A vaccine, the intracellular cytokine responses by CD4+ and CD8+ T cells to AA-ca, Len/47-ca and Len/17-ca and their respective wild-type parental viruses were compared in mice. Day 5 after infection with Len/17-ca, when levels of IL-2, -4 and -10 were highest in the mediastinal lymph nodes (MLN) and lungs, was chosen as the optimum time to harvest lymphocytes and 72 h was determined to be the optimum re-stimulation period for lymphocytes by APCs. Under these conditions, the frequency of CD4+ and CD8+ cells expressing cytokines was highest in the lungs compared with the MLN. A dominant IL-6 response was induced, although all virus strains induced a Th1/Th2 cytokine profile. While the CD8+ cytokine response appeared non-specific, the cytokine response elicited in the lungs by CD4+ cells to Len/17-ca-inoculation was greater than that induced by Len/47-ca, or AA/ca. The CD4+ cytokine response in the lungs may be a useful measure of immunogenicity to determine the most effective influenza reassortant for inclusion in vaccines.
DOI: 10.1016/j.vaccine.2004.10.051
PubMed: 15963362
Affiliations:
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Harrison</name></author><author><name sortKey="Tannock, Gregory A" sort="Tannock, Gregory A" uniqKey="Tannock G" first="Gregory A" last="Tannock">Gregory A. 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Wareing</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology and Environmental Biology, RMIT University, P.O. Box 71, Bundoora, Vic. 3083, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biotechnology and Environmental Biology, RMIT University, P.O. Box 71, Bundoora, Vic. 3083</wicri:regionArea><wicri:noRegion>Vic. 3083</wicri:noRegion></affiliation></author><author><name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C" last="Harrison">Leonard C. Harrison</name></author><author><name sortKey="Tannock, Gregory A" sort="Tannock, Gregory A" uniqKey="Tannock G" first="Gregory A" last="Tannock">Gregory A. Tannock</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>CD4-Positive T-Lymphocytes (immunology)</term><term>CD8-Positive T-Lymphocytes (immunology)</term><term>Cold Temperature</term><term>Cytokines (analysis)</term><term>Influenza A virus (immunology)</term><term>Influenza Vaccines (immunology)</term><term>Lung (immunology)</term><term>Lymph Nodes (immunology)</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Orthomyxoviridae Infections (prevention & control)</term><term>Species Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Cytokines (analyse)</term><term>Infections à Orthomyxoviridae ()</term><term>Lymphocytes T CD4+ (immunologie)</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Mâle</term><term>Noeuds lymphatiques (immunologie)</term><term>Poumon (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité d'espèce</term><term>Vaccins antigrippaux (immunologie)</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T CD4+</term><term>Lymphocytes T CD8+</term><term>Noeuds lymphatiques</term><term>Poumon</term><term>Vaccins antigrippaux</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term><term>CD8-Positive T-Lymphocytes</term><term>Influenza A virus</term><term>Influenza Vaccines</term><term>Lung</term><term>Lymph Nodes</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cold Temperature</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Infections à Orthomyxoviridae</term><term>Mâle</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité d'espèce</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We previously described differences in the 50% protective dose and isotype-specific antibody secreting cell (ASC) responses to US and Russian influenza A cold-adapted (ca) donor strains in the lungs of BALB/c mice [Wareing MD, Watson JM, Brooks MJ, Tannock GA. Immunogenic and isotype-specific responses to Russian and US cold-adapted influenza A vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57, and A/Ann Arbor/6/60 (H2N2) in mice. J Med Virol 2001;65(1):171-7]. A/Leningrad/134/17/57(Len/17-ca) was shown to be a superior immunogen to A/Leningrad/134/47/57-ca (Len/47-ca), which, in turn, was superior to A/Ann Arbor/6/60-ca (AA-ca) but no other comparative data exist. In order to extend our findings and determine a means for selecting the most immunogenic ca influenza A vaccine, the intracellular cytokine responses by CD4+ and CD8+ T cells to AA-ca, Len/47-ca and Len/17-ca and their respective wild-type parental viruses were compared in mice. Day 5 after infection with Len/17-ca, when levels of IL-2, -4 and -10 were highest in the mediastinal lymph nodes (MLN) and lungs, was chosen as the optimum time to harvest lymphocytes and 72 h was determined to be the optimum re-stimulation period for lymphocytes by APCs. Under these conditions, the frequency of CD4+ and CD8+ cells expressing cytokines was highest in the lungs compared with the MLN. A dominant IL-6 response was induced, although all virus strains induced a Th1/Th2 cytokine profile. While the CD8+ cytokine response appeared non-specific, the cytokine response elicited in the lungs by CD4+ cells to Len/17-ca-inoculation was greater than that induced by Len/47-ca, or AA/ca. The CD4+ cytokine response in the lungs may be a useful measure of immunogenicity to determine the most effective influenza reassortant for inclusion in vaccines.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><noCountry><name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C" last="Harrison">Leonard C. Harrison</name><name sortKey="Tannock, Gregory A" sort="Tannock, Gregory A" uniqKey="Tannock G" first="Gregory A" last="Tannock">Gregory A. Tannock</name></noCountry><country name="Australie"><noRegion><name sortKey="Wareing, Mark D" sort="Wareing, Mark D" uniqKey="Wareing M" first="Mark D" last="Wareing">Mark D. Wareing</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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